• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

What effect will a change in temperature of hydrogen peroxide (C) have on the activity of the enzyme Catalase in potatoes measured by the height of the bubbles created by the reaction in a test tube?

Extracts from this document...


Design your Lab: Action of an Enzyme Problem: What effect will a change in temperature of hydrogen peroxide (�C) have on the activity of the enzyme Catalase in potatoes measured by the height of the bubbles created by the reaction in a test tube? Hypothesis: All enzymes either breakdowns substances and\or they put substances together. An enzyme is a protein, folded into a complex three-dimensional shape. The active site is the part of the enzyme that allows it to be a catalyst and where substrates join and react. Anabolic reactions speed up the reactions were large molecules are built up from smaller molecules. This reaction usually needs an input of energy before reaction called the energy of activation. Catabolic reactions speed up the process of breaking down large molecules into smaller molecules. Catalase is a biological catalyst that helps speed up the breakdown of hydrogen peroxide into water and oxygen. Catalase is an enzyme and is the fastest enzyme known. In fact one molecule of Catalase can deal with six million molecules of hydrogen peroxide in 1 minute. The reaction we will be testing is catabolic which also means the reaction will give off some energy. Hydrogen Peroxide is often formed as a product of reactions in cells. It can be poisonous if it builds up, so Catalase has to work quickly so it does not build up to much poisoning the cells and killing them. ...read more.


* Knifes and cutlery * Ice * Cutting tile * Weighting scale * Weigh boat * gloves * goggles * lab coat * water * ruler * hot plate * 6 beakers * Graduated cylinder * Thermometer * Scoopula * Forceps Safety: Hydrogen peroxide is a toxic substance so handle with care and caution. Hot plate will be used to heat water, around these areas use caution. There will be a waste disposal breaker provide for solutions after reactions. Don't pour potato chunks or solution down the sink. Remember to wash equipment before and after each trail. Procedure: 1. Using a knife and other cutlery, cut 6 identical pieces of potato, and weigh each piece to be sure each of them are identical. Record the average weight of the pieces and the temperature of the potato and any qualitative observations. 2. Measure 5ml of hydrogen peroxide using the granulated cylinder and pour into each of the 6 test tube and place test tubes in the rack. Record the temperature of the hydrogen peroxide as this will be the starting point. Tape the height of the liquid in the test tube with a piece of tape. 3. Prepare water and \or ice bath in varying temperature in the 6 different beakers. 0�C, 10�C, 20�C, 30�C, 40�C, 50�C should be the approximate temperatures of the beakers. 4. Place one test tube in each of the water baths. ...read more.


The hypothesis was partially verified but falsified as the enzyme still increasing produced oxygen even after the inferred optimum temperature. In further experiments, the enzyme should be either heat or checked if in fact all the enzymes or the entire piece was in fact the same temperature as the manipulated temperature being tested. The background information relived on the enzyme being heat but in this experiment it was most likely shielded from the high heat in the cores of the potato samples. The samples of the potatoes were not identical and produced irregular results. Maybe if there was a machine where it could create identical pieces using automation but even if the size and shape were the same we can never be sure if the number of Catalase would be identical. A three digit scale could have been used. The samples could have been used as a liquid by crushing the potatoes or using a blender to ensure uniform samples. The experiment could have use a vacuum to collect the oxygen as the bubbles could have burst releasing oxygen and losing it form the system. The experiment should have been repeated 7 times or trails for accuracy but there was limited time and only individual work. Synthesis: The heating of the Substrate molecules instead (hydrogen peroxide) and this showed that with increasing temperatures of the substrate molecule the enzymes reacted quicker. This occurred because as the substrate molecules were heated they moved around more increasing the chance of them having a successful collision and reacting with the Catalase. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. The effect of concentration of sucrose solution on the osmosis in potato

    Some have decrease in mass and others have increase in mass. There may be some error when using the electronic balance, e.g. the cover has not been placed on during the measurement. 3.3 Improving the Investigations Table 6: Suggestions for Improvement Limitation identified Type of resultant error (ie.

  2. Browning Enzyme

    Using them ensure that all the discs are of the same size. * I do not need much solution and so using a small 10 ml measuring cylinder rather than a large one means a small uncertainty to deal with.

  1. Isotonic Point of Potatoes

    in each petri dish of a certain glucose concentration. 6) After doing so, cover these petri dishes and place them in a corner, safe from any type of interference. 7) After about a day, remove all these samples from the solutions, reweigh and re-measure the mass and length of the potato strips from each sample respectively.

  2. Enzyme activity using the enzyme catalase in different plant sources

    Put the test tubes into the water bath 11) Start the stopwatch 12) After 10 minutes check your test tubes 13) After 20 minutes, remove the test tubes and place it back into the test tubes racks 14) Record the height of the foam immediately 15) Rinse all the materials that won't be used to keep work station clean 16)

  1. Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

    Further investigations could investigate other possible limiting factors, such as temperature, pH, or concentration of enzyme. To help make this experiment more accurate, I repeated it three times and then used the average of all the results to plot a graph with a line of best fit.

  2. In this extended essay I am looking at the effect of different kind of ...

    It is a lengthy process, though. How music affects plant growth, cannot be observed within days of the plant being exposed to music. It takes few weeks to identify the effects of music on plants. Now talking about Chickpea, it is also known as gram seeds. This is an angiosperm.

  1. Experiment-Effect of Temperature on the Action of Amylase Enzyme

    The mixture was very concentrated which can be seen obviously from their dark colors. Although the wavelength used was 620 nm which is quite high but it not enough to penetrate the molecules of the high concetrated mixture. This, thus, may be responsible for the identical duplicate results in rate

  2. The Effect of Temperature on the Vitamin C Content of Lemon Juice

    Next time we would use distilled water to dilute our lemon juice. To add the lemon juice we had to hold the test tube outside the water bath so we could visually monitor the colour change of the solution. This meat the temperature of the lemon juice and the DCPIP cooled while we were adding the lemon juice.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work