Introduction to Spectrophotometric Analysis - By using spectrophotometric analysis or spectrophotometry, one can determine the identity in terms of structure and species of a biomolecule as well as establish the concentration of a certain biomolecule

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Name: Ng Yen Pheng

Student ID: 22353046

Date: 6 March 2012

Demo:

Title: Introduction to Spectrophotometric Analysis

Introduction:

Spectrophotometric Analysis which is one of the most common and valuable analysis techniques used to study the interaction of electromagnetic radiation with chemical compounds (Stewart & Ebel, 2000). By using spectrophotometric analysis or spectrophotometry, one can determine the identity in terms of structure and species of a biomolecule as well as establish the concentration of a certain biomolecule (Stewart & Ebel, 2000).

Absorption spectrophotometry is the most common of spectrophotometry technique used by biochemist. The science behind this technique is when light passes through a solution, components or substances in the solution will absorb some of the light that passes through. This will result in the excitation of an electron to a higher energy level, and when the electron loses its energy and returns to its original energy state, the energy that is released is shown as emission of light in a different wavelength which results in a different colour, this process is known as fluorescence (Stewart & Ebel, 2000).

Beer-Lambert`s Law describe the relationship between the substance concentration and