>Peel the fruit, as there are barely any enzymes in the peel.
>Chop the fruit.
>Place a small amount in a homogeniser using 50mls buffer to blend.
>Filter blended mix to remove solid debris.
>Take 5 petri dishes with solid gelatin in.
>Use a no. 7 cork borer to make 3 holes in each dish.
>Ensure holes are evenly spaced and not too close to the side of the dish.
>Fill each of the holes in the dishes with a different solution.
Dish 1 - 3 holes with 100%
Dish 2 - 3 holes with 50%
Dish 3 - 3 holes with 25%
Dish 4 - 3 holes with 12.5%
Dish 5 - 3 holes with 0%
Prediction
Pineapple is thought to contain protease, so using this I predict that the protease will digest the protein in the gelatin, leaving behind and amino acid solution. Also I predict that the dishes with the more concentrated solutions will have a larger amount of digestion, and the dish with 0% concentration will have no change. Aswell as knowing that the protease will digest the gelatin, I can prove that by the fact that you cannot put pineapple pieces into gelatin jelly because then the jelly does not set properly. This is due to the enzymes in the pineapple.
Analysis
This shows that although there was anomalous results, my prediction was correct, and that the highest concentration of solution had the most difference.
This happened because the protease in the pineapple solution digested the protein in the gelatin, leaving behind a small amount of liquid, which was amino acid.
Lizzi Ring 10NCS
