Kristin Quinn
Bioinorganic Coordination Chemistry: Copper (II) Tetraphenylporphyrinate
February 17, 2005
Quinn 1
Kristin Quinn
Intermediate Inorganic Lab
17 February 2005
Abstract
This experiment uses H2TTP made during the last lab and hydrated copper (II) acetate to convert the H2TTP to Cu(TTP). The reaction proceeds by being refluxed for 30 minutes. The final product is a non-iridescent purple color, unlike the vivid, shimmering purple color of the starting H2TTP.
Introduction
Thin layer chromatography, or TLC, is used as a prerequisite for column chromatography. When performing types of chromatography, like TLC, the polarity of solvents is extremely important. This lab uses TLC plates, five solvents (hexane, toluene, ethyl acetate, dichloromethane, and acetone), and small evaporating dishes to determine which solvent would be best to use in column chromatography. The least polar solvent is hexane, which has a polarity index of 0.1. Toluene has a polarity index of 2.4, while ethyl acetate’s is almost twice that at 4.4. By comparing the polarity indexes, you can tell what solvent will separate your dots of product on your TLC plates the best (Skoog 761).
Experimental Section
The equipment needed for this experiment is as following: 100 mL round bottom flask, stir bar, water condenser, hot plate, UV light, Pasteur pipet, ice bath, separatory funnel, rotary evaporator, evaporating dish, cotton for filtering, and UV-vis spectrometer.
Quinn 2
The chemicals required are: 0.1 g. of H2TTP made previously, 20 mL N,N-dimethylformamide, 0.16 g. hydrated copper acetate, distilled water, 75 mL dichloromethane, hexane, toluene, ethyl acetate, dichloromethane, and acetone for TLC plates.