Method:
- The factor of this experiment that I am going to be changing is the temperature of the water, and thus the temperature of the plants surroundings.
- To increase the temperature of the water, I will simply place a Bunsen Burner underneath the beaker in which the plant is, and wait until the temperature of the water is the desired one.
- The temperature of the water will remain at the same level for quite a long time; long enough for me to carry out my readings. This is because I will fill the beaker with a large volume of water, which will mean that the heat will not escape from the water very easily. And so any time I will want to increase the temperature I will just have to simply place the Bunsen underneath until it reaches the temperature and then remove the Bunsen and so on.
- In order to measure the temperature, I will place a thermometer in the boiling tube that the plant is in. I intend to start at 0ºC and then work my way up to around 60ºC, taking measurements at 5ºC intervals. I will take the recordings of the 0ºC experiment first as then this enables me to gradually increase the temperature of the water, which is much easier than having to cool the water down.
- During my experiment, I intend to measure the amount of oxygen given off per minute by the pondweed plant by collecting it in a measuring cylinder. Another way to do this would be to count how many bubbles the plant produces in a certain time, but that is slightly less accurate and so I have decided to do this instead. Also, there is a chance of a human error where I may miss a bubble being produced, so I have decided to use the other method instead. I intend to change the temperature and then see what impact that has on the amount of oxygen produced, and so thus the rate of photosynthesis.
- I will collect the oxygen in a measuring cylinder, as the oxygen will displace the water in the tube and so I will be able to have an accurate recording of how much was given off. To make my results as reliable as possible, I am going to repeat each reading 3 times, and so thus take a total of 36 recordings
- In this experiment, I will need to keep three things at the same level. These three things are light intensity, carbon dioxide levels and the volume of the water.
- To keep the light intensity constant, I intend to place a lamp at exactly 20cm away from the plant each time to ensure the light the plant receives each recording will be the same and so not change the result. To keep the water level the same, I will simply fill the beaker of water up to a certain point each time, say 850ml. And to keep the carbon dioxide levels the same, each time I repeat the experiment I will replace the water inside the boiling tube with fresh water, and place 5 drops of sodium hydrogen carbonate in the water each time, as this will ensure that each plant will have the same level of carbon dioxide by it. By doing these things every experiment, I will ensure that my investigation is a fair one. However, it may not be possible to keep the light intensity constant, as at different times of the day there will be different amounts of sunlight reaching my plant, and so this may have an impact on the light intensity of the investigation. However, this impact will be minimal, so I have no worry about this threat to my experiment.
Safety Considerations – There are some important things I will have to bear in mind whilst carrying out this experiment. First of all, the Bunsen Burner is very dangerous, and can cause serious damage should the flame touch the body. Therefore, I am going to tuck in my tie to prevent that from catching fire, and I am going to wear goggles just in case some flickers of the flame come towards me. It is important to remain standing whilst carrying out the experiment so that if I need to quickly get out of the way, I am actually able to do so. Another safety consideration is the sodium hydrogen carbonate. If it gets onto the skin, it must be washed out immediately with soap and water, as it is an irritant and could cause damage to sensitive skin.
The information I obtained for calculating my prediction was mainly from my school workbook, but also from my textbook “Biology Lives” by Morton Jenkins and from another textbook “Revise GCSE Biology” by Letts.
Apparatus – The apparatus I will be using for my investigation is shown below.
Before I carried out my major experiment, I decided to take some trial results just to see if I needed to change anything in my method.
Here are the results I obtained, using light from 20cm away and putting in 5 drops of sodium hydrogen carbonate:
Method Redraft
I soon realised that there was a serious flaw with a part of my method. There is not enough oxygen produced in one minute to measure accurately using a measuring cylinder. I seriously doubted that this situation would change if I was to extend the experiment to two minutes either, and so I had to come up with another idea. I decided to try my other method, which was to count the number of bubbles produced in a minute. This was much more effective and I was able to gather some solid results which seemed to be relatively close to each other. Also, I found that when I was trying to keep the pondweed underwater in the boiling tube, I struggled a fair amount. So then I decided to come up with another idea, which was to attach plastercine or blue-tack to the side of the boiling tube which would then hold the plant in place. I also realised that I should place the pondweed upside down in the boiling tube, so that the maximum amount of carbon dioxide could be released efficiently from the plant, as the underside of the plant is where the stomata are, and this is where the gases leave and enter the plant. So, a redrawn diagram of my apparatus has been included underneath.
Modifications – After carrying out the experiment, I realised my method was not going to be able to be carried out. I stated that I would start recording at 0ºC and then work my way up to about 60ºC. In the investigation, I was able to go up to 60ºC but I was unable to start my recording at 0ºC. In the end, I had to start at 20ºC. This was because even after putting in lots of ice, the temperature was taking a long time to decrease, and due to the time constraints upon me, I decided to start at 20ºC. This was a good choice as I was able to then obtain 3 results for temperatures increasing by 5ºC each time up to 60ºC. Also, I decided to place the lamp right next to the beaker, not 20cm away from the beaker as I had originally planned. This had to be done because I realized that the time I was given meant that I had to get the plant producing bubbles quickly, and so I could not risk wasting time waiting for the plant to do so. I moved the lamp right next to the beaker so that would hopefully speed up the rate of photosynthesis and thus the rate that oxygen was produced. It was successful and I was able to obtain my results in time.