• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The Effect of Temperature on Enzymatic Activity. Aim To investigate enzyme activity of yeast on glucose at different temperatures

Extracts from this document...


Effect of Temperature on enzyme activity Aim To investigate enzyme activity of yeast on glucose at different temperatures Hypothesis As the temperature increases, enzyme activity will also increase. At lower temperatures enzyme activity will be less than when the temperature is higher. Also if temperature is increased too much, enzymatic activity will fall. This is because the molecules in glucose will move faster and the enzymes will be have increased activity because of increased movement. Also since all enzymes have an optimum temperature at which enzymatic activity is the highest, increasing the temperature will move enzyme closer to optimum temperature. But if temperature is increased too much, enzyme will start to denature (loss of three-dimensional structure) and thus the enzymatic activity will fall again Key Variables Independent: Temperature Dependent: Enzymatic activity (number of bubbles) Controlled: Amount of water that the solution is submerged in Time Concentration of glucose Materials � � 100mL beakers x5 (label from A-E) ...read more.


and record the number of bubbles produced in 10,15 and 20 minutes 8. 8. Set up a hotplate at 30�C 9. 9. Fill Beaker C with 80 mL of water and place on hotplate 10.Fill 3cm3 of glucose solution into the syringe and then in the same syringe insert 2 cm3 of the yeast solution 11.Place the syringe into Beaker C and record the number of bubbles produced in 10,15 and 20 minutes 12.Repeat steps 9-11 for 40�C and 50�C in Beaker D and E Data Raw Data Time 10�C 20* C 30*C 40*C 50*C 10 3 7 15 36 18 15 4 9 20 42 18 20 6 11 24 45 20 Processed Data Time (minutes) Temperature 10�C 20* C 30*C 40*C 50*C 10 3 7 15 36 18 15 4 9 20 42 18 20 6 11 24 45 20 Data Analysis From the data above it can be seen that the hypothesis was correct because the enzymatic activity did increase until the optimum temperature of 40�C was reached and started to fall after that point. ...read more.


Evaluation Limitations Evaluations The intervals between our temperatures was 10 degrees which is relatively inaccurate whereas we could have used a smaller gap between temperatures to gain more accuracy Next time, I would use 2 degree gaps between my temperatures so I would have a more specific set of data and a smoother curve for the data. The method in which we measured the enzymatic activity was very inaccurate as a result of human error because we manually counted the bubbles as we saw it. This was a very crude and inaccurate way of measuring because there were points were many bubbles came out at once so it was hard to count them all and there were many small ones attached to big ones so a few bubbles might have been counted twice or not counted at all. Since there is not many ways in which this error can be improved, we could use another factor to measure enzymatic activity such as the pH level. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a teacher thought of this essay

3 star(s)

A sound report on the effect of various temperatures on the action of enzymes.

The hypothesis was sound but the introduction would have benefited from some more scientific theory on the yeast and glucose and why the CO2 is being produced.

The method is good. The reader is able to repeat the experiment which is a measure of an acceptable method. The procedure could be improved in the parts that I have commented on.

The data would have benefited from repeats so that averages could be calculated.
A good conclusion and evaluation.

Marked by teacher Jon Borrell 22/05/2013

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Marked by a teacher

    The effect of Pectinase Concentraton on the production of apple juice

    Extreme temperatures may also denature enzymes or slow down the reaction, so keeping a constant temperature will ensure that this will not happen. * Volume of Pectinase - this is important to keep constant because it ensures that the results are fair, as different volumes of Pectinase will give the

  2. affect of age on reaction time

    Get the material used (ruler). 3. Hold the ruler vertically from the end of the ruler (45 cm). 4. Then let the volunteer spread his thumb and middle finger 8.75 cm apart and centre the ruler between his fingers.

  1. Browning Enzyme

    The enzymes contain copper at their active site. This metal ion enables them to oxidize the phenolic group.2 Moreover, the substrate for the enzyme is polyphenols and it is a phenolic compound. The reaction that occurs as a result of the presence of oxygen is called enzymatic browning and it

  2. The Effect of Glucose Concentration on Anaerobic Respiration in Yeast

    When the concentration of glucose was 10%, the amount of bubbles produced is relatively low, only 26 in 5 minutes. The difference between the amount of bubbles produced at 10% sugar concentration and 30% sugar concentration is visible. (103 bubbles in 5 minutes at 10% concentration).

  1. Isotonic Point of Potatoes

    Cut 5 potato strips for each sample into the same size as the other strips in the same sample. 3) Measure the length and mass for each of these potato strips for all of the 5 samples using the electronic balance (+/- 0.01g)

  2. To investigate how aerobic exercise affects the heart rate

    Method 1. Measure resting heart rate (before exercise) using an automatic blood pressure monitor 2. Perform jumping jacks for amount of time indicated 3. Immediately measure pulse using the blood pressure monitor 4. Record pulse rate in a table 5.

  1. Vitamin C concentration

    * 2 pipettes (10cm3 �0.5) * Pipette (1cm3 �0.05) * DCPIP solution * Ascorbic acid solution (concentration 0.1%, 500cm3 �1) * Bottle of distilled water * 8 filters * Fruit press * dropper Method: 1. Label the test tubes, then slowly pipette 1 cm3 of DCPIP into each test tube.

  2. Experiment-Effect of Temperature on the Action of Amylase Enzyme

    The rate of amylase enzyme activity was the highest if it is closest to 37°C which is equal to human body temperature as well as the optimum temperature for enzymes to operate. Beyond the temperature, the rate of amylase enzyme activity will decrease gradually due to denaturation of the amylase enzyme.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work