Temperature of Hydrogen Peroxide on Speed of Decomposition Reaction of Chicken Liver

Problem: What effect does the temperature of hydrogen peroxide, in degrees Celsius have on the speed of the decomposing the chicken live and produce oxygen and hydrogen, measured in terms of the height of the bubbles produced in centimetres(cm)?

Background Information: Hydrogen peroxide is a by product of many reactions in our body. When enzymes break down amino acids and fatty acids they make large amounts of hydrogen peroxide. High amount of hydrogen peroxide can cause chemical burns and tissue damage. Catalase is a common enzyme found in most living organisms. Catalase is frequently used by cells to rapidly catalyze the decomposition of hydrogen peroxide into less reactive gaseous oxygen and water molecules. Biological catalyst is usually found in high concentrations in the liver. It catalyzes the decomposition of hydrogen peroxide into oxygen and water. The optimum temperature for it is 37oC and once the temperature is above 40oC, the catalase begins to denature and eventually coagulates. Also, for every 10°C increase in temperature, there is a double in the rate of reactions of enzymes within a certain range.

Prediction: With every increase of 10oC, the height of bubbles produced will double.

Hypothesis: The higher the temperature of the hydrogen peroxide, the faster the molecules in it move, therefore, when the temperature increases, there is a higher chance for the substrate (hydrogen peroxide) to collide with the enzyme. Therefore, more bubbles to be created when heated and visa versa for when cooled.

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Experimental Design: The chicken liver will be cut up into 15 identical 0.5g pieces and each piece would have to be put in a separate test tube. There will be another 15 test tubes, each containing one millilitre of hydrogen peroxide. The test tubes will be split into 3 groups of 5 and each group will be heated or cooled to a different temperature (7oC, 22oC and 37o). When they reach their respective temperatures, the chicken liver will be carefully put into the test tubes containing the hydrogen peroxide. The height of the bubble will be recorded for every 10, 20 ...

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