The use of pectinase in fruit juice production
The use of pectinase in fruit juice production I predict that there will be no juice produced to the apple sauce that had no enzyme and juice will produce to the apple sauce that had pectinase. A control is carried out in order to compare the rate of reaction between with pectinase and without pectin's. Pectin --> sugars + galataronic acid Pectin is a substance which helps to hold pant cell walls together. As a fruit ripens the plant produces proteolytic enzymes, which convert the insoluble protpectin of the unripe fruit into more soluble forms, causing the fruit to soften. When fruits are mashed and pressed to form juices these more soluble forms of pectin enter the juice, making it cloudy and causing the colour and flavour to deteriorate. Enzymes are specific in the reactions they catalyse, much more so than inorganic Catalysts. Normally, a given enzyme will Catalyse only one reaction, or type of reaction. The enzyme has an active site that helps it to recognise its substrate in a very specific way. Just like a key only fits into a specific lock, each enzyme has its own specific lock; each enzyme has its own specific substrate. This is called the lock and key theory. The enzymes never actually get consumed in the process; they just increase the rate of reactions. When enzymes denature the heat starts to destroy their shape and structure. The shape of the enzyme is so
Type of Ownership
Type of Business Ownership 4th October 2008 Types of ownership Sole proprietor- A sole proprietor is a single person owning a business. Sole trader have unlimited liability (all debts paid by the owner, personal possessions may be taken). Partnership- 2 - 20 partners own, control and finance the business. They have unlimited liability. In a partnership the partners need to draw up a 'Deed of Partnership' to verify their company and to describe each partner's role in the business. Private Limited Company (Ltd)- A Company owned by shareholders. A limited number of shares are issued (99); these are owned by family and friends of the business. The business has limited liability (the only money lost is the money already invested in the company such as the shares, set up cost and others. Personal belongings are safe). A lot of small businesses are private. Public Limited Company (PLC)- A Company owned by shareholders, unlimited amount of shareholders. It must have £50,000 of capital, and may allow its shares to be bought by the general public via the stock market. The business has limited liability. Every PLC must send an annual return to Companies House at least once every 12 months. Franchise- Franchising refers to the methods of practicing and using another person's philosophy of business. The franchisor grants the franchisee the right to distribute its products,
The Supernatural in "Macbeth"
Macbeth Supernatural, no matter if you believe in it or not it still attracts the audience's heart. In the time of William Shakespeare there was a strong belief in the existence of the supernatural. Thus, the supernatural is s recurring aspect in many of Mr. Shakespeare's plays. In "Macbeth", the supernatural is an integral part of the structure of the plot. Not only do witches appear but also a floating dagger transforms into reality. Shakespeare intelligently introduced the elements of the supernatural in Act 1, Scene 3. Shakespeare located his scene in a mysterious marshy, deserted "heath" on a day with "thunder", perfect for paranormal activity. Thunder and lightning was believed to be associated with evil, and when the play was presented in Shakespeare's time, cannonballs would be rolled to create the sound effect. Three characters are introduced; immediately we knew that they are involved with the supernatural because the second witch told the others that she was "killing swine". Witches tried to hide from humans in fear of being burned at stakes, so they had to be in an empty heath to meet Macbeth. Back in the day, witches were blamed for the death of animals. Also, the witches repeated phrases three times. "I'll do, I'll do, I'll do" and instead of any other number of witches, there were three of them. The number three was regarded as a magic number. The
Use of a Redox Indicator to show Dehydrogenase Activity
Use of a Redox Indicator to show Dehydrogenase Activity Hypothesis: As the temperature increases, the time taken for the colour change to occur will decrease. Introduction: Triphenyl tetrazolium chloride (also known as T.T.C) is an example of an artificial hydrogen acceptor. It is a redox indicator which is colourless when oxidised, however when reduced, it produces a red, insoluble precipitate called 'formazans'. T.T.C can therefore be used to investigate the enzyme activity of dehyrogenase enzymes by showing a colour change when they are present. The purpose of this experiment is to see what effect temperature has on the activity of dehydrogenase enzymes within yeast cells. Materials/Apparatus: * Actively respiring yeast suspension. This is prepared by adding 10g of dried yeast to 1dm3 of distilled water, followed by mixing in 50g of glucose. This mixture should be allowed to stand for 24 hours before the experiment takes place. * Tiphenyl tetrazolium chloride is used as a redox indicator to investigate the activity of dehydrogenase enzymes when yeast suspension is exposed to different temperatures. * Distilled water for the preparation of the yeast suspension. * Test tubes to place the mixture of yeast and T.T.C. * Test tube rack to allow the test tubes to stand upright in the water baths. * Incubator to allow enzyme activity to occur at different temperatures *
What is the importance of the land in Twentieth Century Irish Poetry?
What is the importance of the land in Twentieth Century Irish Poetry? Land in the Twentieth Century was very important to the Irish nation, and this is portrayed through the works of certain pieces of poetry, written by native countrymen Thomas Kinsella and Seamus Heaney. The poem 'Wormwood' is expressed by Thomas Kinsella in a powerful and descriptive manner where the reader can experience the deepest thoughts of the writer, in his or her own way. The reader feels a sense of involvement as Kinsella sets the scene in the dank woods: "In a thicket, among wet trees, stunned, minutely Shuddering, hearing a wooden echo escape." Kinsella informs us of a tree, which he is in fact bewildered by. How he has never come across a tree like this before. It has a certain grace and elegance due to its individuality. The sheer size of the tree he finds mesmerizing, and describing the slenderness of how the tree appears to the naked eye: "The two trees in their infinitesimal dance of growth Have turned completely about one another, their join A slowly twisted scar..." Then Kinsella's dreams are shattered, as a kind of axe breaks the bond between these two trees. As this axe shatters the tree it also shatters the dreams of Kinsella: "A wooden stroke: Iron sinks in the gasping core. I will dream it again." Wormwood was one of Kinsella's poems which he wrote during the twentieth
